+Marker Data: AFS015

Crop bunching_onion
Population DJF2
Linkage Group Chr1a
Marker Type SSR
Map Position 32.7
Annotation
Source Name AB499317
Source Type genomic
Source Sequence
Developer NIVTS
Document Ohara et al. (2005) Euphytica 144:255-263
motif (ta)4tgtatg(ta)6tg(ta)5tgtg(ta)6tg(ta)3
PCR product (bp) 344
fwd primer AFS015-F
fwd primer seq. atctcactgtccttgtacctgaaag
rev primer AFS015-R
rev primer seq. catcttgactttgtgatatttgtgc
fluor. primer AFS015-F(FAM)
dye FAM
fluor. Primer seq. FAM-atctcactgtccttgtacctgaaag
PCR condition 94C_2min->(94C_30s->60C_30s,-1C/cyc->72C_30s)x10->(94C_30s->50C_30s->72C_30s)x35->72C_4min->10C
polymerase Toyobo rTaq DNA polymerase
Genebank No. AB499317
annotation
similar marker
product size in J 330
product size in Kaizuka-Wase
product size in Iki-White 270
product size in Tender Pole
Line allele-1 allele-2
C1s-11s-25s-7 360 360
Cho1s-1s-2s-2s-8s 360 360
D1s-15s-10s-8 360 360
F1s-7s-17s-3s-1 330 360
J1s-14s-23s-9 330 330
K1s-5s-2s-2s-4s-5 360 360
Chiang_mai 260 260
Kaizuka_Wase
Population Linkage Group Position
DJF2 Chr1a 32.7
P1 J02 19.0
P2 D02 9.2
DB Program Definition E-Value
EST Name Species Strain