+Marker Data: AFRA07E10

Crop bunching_onion
Population DJF2
Linkage Group Chr4a
Marker Type SSR
Map Position 40.3
Annotation
Source Name AB499519
Source Type genomic
Source Sequence
Developer NIVTS
Document Tsukazaki et al. (2007) Euphytica 157:83-94
motif (tc)9
PCR product (bp) 168
fwd primer AFRA07E10-U
fwd primer seq. atgaactagacggtttgggaggaa
rev primer AFRA07E10-L
rev primer seq. gtttagctcatattttgaagctcgcagg
fluor. primer AFRA07E10-F(FAM)
dye FAM
fluor. Primer seq. FAM-atgaactagacggtttgggaggaa
PCR condition 94C_2min->(94C_30s->65C_30s,-1C/cyc->72C_30s)x10->(94C_30s->55C_30s->72C_30s)x35->72C_4min->10C
polymerase Toyobo rTaq DNA polymerase
Genebank No. AB499519
annotation
similar marker
product size in J 180
product size in Kaizuka-Wase 190
product size in Iki-White 210
product size in Tender Pole
Line allele-1 allele-2
C1s-11s-25s-7 172 172
Cho1s-1s-2s-2s-8s 172 172
D1s-15s-10s-8 172 172
F1s-7s-17s-3s-1 170 170
J1s-14s-23s-9 178 178
K1s-5s-2s-2s-4s-5 170 170
Chiang_mai 219 219
Kaizuka_Wase
Population Linkage Group Position
DJF2 Chr4a 40.3
KiCF2 4a 99.5
DB Program Definition E-Value
EST Name Species Strain