motif |
(tg)5cagttgta(tg)8cgcgtgcatgcgtgcgtgtgcatgc(gt)3atgtgctac(tg)3ca(tg)4cagttgtgta(tg)5catagttgta(tg)7
|
PCR product (bp) |
292
|
fwd primer |
AFRA04B10-U
|
fwd primer seq. |
atgagtgtgcagttttgtgagggt
|
rev primer |
AFRA04B10-L
|
rev primer seq. |
gtttgcgcaggtccatctgtaaaaa
|
fluor. primer |
AFRA04B10-F(NED)
|
dye |
NED
|
fluor. Primer seq. |
NED-atgagtgtgcagttttgtgagggt
|
PCR condition |
94C_2min->(94C_30s->65C_30s,-1C/cyc->72C_30s)x10->(94C_30s->55C_30s->72C_30s)x35->72C_4min->10C
|
polymerase |
Toyobo rTaq DNA polymerase
|
Genebank No. |
AB499515
|
annotation |
|
similar marker |
|
product size in J |
300
|
product size in Kaizuka-Wase |
|
product size in Iki-White |
|
product size in Tender Pole |
|