| motif |
(ag)8(tg)10(ag)8cgtacttgt(ta)3ttttcattttcaaaac(ta)3ttattatatt(ta)4
|
| PCR product (bp) |
221
|
| fwd primer |
AFHT11E07-U
|
| fwd primer seq. |
aggtgagcttaggttgcgttgttt
|
| rev primer |
AFHT11E07-L
|
| rev primer seq. |
gtttcaagtagtacccgttggtgcagtg
|
| fluor. primer |
|
| dye |
|
| fluor. Primer seq. |
|
| PCR condition |
94C_2min->(94C_30s->65C_30s,-1C/cyc->72C_30s)x10->(94C_30s->55C_30s->72C_30s)x35->72C_4min->10C
|
| polymerase |
Toyobo rTaq DNA polymerase
|
| Genebank No. |
AB499508
|
| annotation |
|
| similar marker |
|
| product size in J |
230
|
| product size in Kaizuka-Wase |
180+190
|
| product size in Iki-White |
200
|
| product size in Tender Pole |
200
|
